The IEEE International Symposium on Biomedical Imaging (ISBI) is the premier forum for the presentation of technological advances in theoretical and applied biomedical imaging. ISBI 2016 will be the thirteenth meeting in this series. The previous meetings have played a leading role in facilitating interaction between researchers in medical and biological imaging. The 2016 meeting will continue this tradition of fostering crossfertilization among different imaging communities and contributing to an integrative approach to biomedical imaging across all scales of observation. The meeting will be held in Prague, Czech republic, between 13 – 16 April 2016.